Biochemistry

Chapter 6: DNA & Biotechnology

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In eukaryotes, DNA is wound around histone proteins to form ____ .

In eukaryotes, DNA is wound around ____ to form nucleosomes .

In eukaryotes, ____ is wound around histone proteins to form nucleosomes .

Histone proteins that DNA is wound around are ____.

Nucleosomes, may be stabilized by another histone protein (____).

As a whole, DNA and its associated histones make up ____ in the nucleus.

As a whole, DNA and its associated histones make up chromatin in the ____.

____ is dense, transcriptionally silent DNA that appears dark under light microscopy.

Heterochromatin is ____ under light microscopy.

Euchromatin is ____ under light microscopy.

____ is less dense, transcriptionally active DNA that appears light under light microscopy.

____ are the ends of chromosomes.

Telomeres are the ends of ____.

Telomeres contain high ____-content to prevent unraveling of the DNA.

____ contain high GC-content to prevent unraveling of the DNA.

Telomeres contain high GC-content to prevent ____ of the DNA.

During replication, ____

____ are located in the middle of chromosomes.

____ hold sister chromatids together until they are separated during anaphase in mitosis.

Centromeres hold ____ together until they are separated during anaphase in mitosis.

Centromeres hold sister chromatids together until they are separated during ____ in mitosis.

Centromeres contain a high ____-content to maintain a strong bond between chromatids.

Centromeres contain a high GC-content to ____.

The ____ is a set of specialized proteins that assist the DNA polymerases.

The replisome (replication complex) is a set of specialized proteins that assist the ____.

To replicate DNA, it is first unwound at an origin of replication by ____.

To replicate DNA, it is first unwound at an ____ by helicases.

Unwounding at origin of replication produces two ____ on either side of the origin.

Prokaryotes have ____ origin(s) of replication.

____ have a circular chromosome(s) that contain only one origin(s) of replication.

____ have linear chromosome(s) that contain many origin(s) of replication.

Eukaryotes have ____ origin(s) of replication.

Unwound strands are kept from reannealing or being degraded by ____.

____ causes torsional strain on the DNA molecule.

Supercoiling causes ____ on the DNA molecule.

Supercoiling can be released by ____.

____ can be released by DNA topoisomerases.

DNA topoisomerases function by ____.

Type ____ topoisomerases relax DNA (i.e., remove supercoils ) by nicking and closing one strand of duplex DNA.

Type I topoisomerases ____ ) by nicking and closing one strand of duplex DNA.

Type I topoisomerases relax DNA (i.e., remove supercoils ) by ____.

Type ____ topoisomerases change topology of DNA by breaking and rejoining double-stranded DNA .

Type II topoisomerases ____ .

DNA replication is ____ .

Semiconservative DNA means ____.

DNA cannot be synthesized without an adjacent nucleotide to hook onto, so a ____.

DNA polymerase ____ is found in prokaryotes.

DNA polymerase III is found in ____.

DNA polymerases α, δ , and ε are found in ____.

DNA polymerases ____ are found in eukaryotes.

DNA polymerases read the template DNA ____′ to {{1::3}}′.

The ____ strand requires only one primer and can then be synthesized continuously in its entirety.

The leading strand requires only ____ primer and can then be synthesized continuously in its entirety.

The ____ strand requires many primers and is synthesized in discrete sections called Okazaki fragments.

The lagging strand requires many primers and is synthesized in discrete sections called ____.

RNA primers can later be removed by ____ (eukaryotes).

RNA primers can later be removed by DNA polymerase I (____).

RNA primer place is filled in with DNA by ____ (eukaryotes).

RNA primer place is filled in with DNA by DNA polymerase I (____).

____ can fuse the DNA strands together to create one complete molecule.

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