Biochemistry

Chapter 6: DNA & Biotechnology

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There are two chemically distinct forms of nucleic acids within eukaryotic cells.____.

The bulk of DNA is found in ____.

Nucleosides are composed of a ____.

____ are composed of a five-carbon sugar bonded to a nitrogenous base.

Nucleosides are formed by covalently linking the ____.

Nucleotides are formed when one or more ____.

____ are formed when one or more phosphate groups are attached to the C-5' of a nucleoside.

Nucleotides in DNA contain ____.

The backbone of DNA is always read from ____′.

DNA backbone is formed as nucleotides are joined by ____ bonds.

Phosphates carry a ____ charge.

DNA and RNA strands have an overall negative charge, because of ____.

DNA and RNA strands have an overall ____ charge, because of the phosphates.

Each strand of DNA has distinct 5′ and 3′ ends, creating ____ within the backbone.

DNA strands run ____ to one another.

Enzymes that replicate and transcribe DNA only work in the ____′ direction.

There are two families of nitrogen-containing bases found in nucleotides: ____.

____ contain two ring(s) in their structure.

Purines contain ____ ring(s) in their structure.

____ contain one ring(s) in their structure.

Pyrimidines contain ____ ring(s) in their structure.

The two purines found in nucleic acids are a____.

The two ____ found in nucleic acids are adenine ( A ) and guanine ( G ).

The three pyrimidines are ____.

The three ____ are cytosine ( C ), thymine ( T ), and uracil ( U ).

While ____ is only found in RNA.

Purines and pyrimidines are examples of biological ____.

In chemistry, the term aromatic describes any unusually stable ring system that adheres to the following four specific rules: ____>

In an aromatic compound, the extra stability is due to the ____.

All six of the carbon atoms in benzene are ____-hybridized.

Heterocycles are ring structures that contain ____ elements in the ring.

DNA is organized according to the ____ model.

In DNA, there are two strands with ____.

____ always pair with pyrimidines (C, U, and T).

Purines (A and G) always pair with ____.

In DNA, A pairs with T (via ____ hydrogen bonds).

In DNA, ____ (via two hydrogen bonds).

In DNA, ____ (via three hydrogen bonds).

In DNA, C pairs with G (via ____ hydrogen bonds).

In RNA, A pairs with U (via ____ hydrogen bonds).

In RNA, ____ (via two hydrogen bonds).

____ state that purines and pyrimidines are equal in number in a DNA molecule.

Chargaff’s rules state ____.

Chargaff’s rules state that because of base-pairing, the amount of ____.

The double helix of most DNA is a ____ .

The helix in B-DNA makes a turn every ____ bases within that span.

____ can be identified between the interlocking strands of DNA backbone.

Major and minor grooves can be identified between the ____ of DNA backbone.

Major and minor grooves are often the site of ____.

Another form of DNA is called Z-DNA for its ____ appearance.

Another form of DNA is called ____ for its zigzag appearance.

Z-DNA is a left-handed helix that has a turn every ____ bases within each turn.

Z-DNA is a ____ helix that has a turn every 4.6 nm and contains 12 bases within each turn.

____-DNA is a left-handed helix that has a turn every 4.6 nm and contains 12 bases within each turn.

A high GC-content or a high salt concentration may contribute to the formation of ____-DNA.

A high ____ may contribute to the formation of Z-DNA.

DNA strands can be pulled apart, ____ .

DNA strands can be brought back together, ____.

____ can cause denaturation of DNA.

DNA is organized into ____ chromosomes in human cells.

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