Biochemistry

Chapter 3: Nonenzymatic Protein Function & Analysis

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Column chromatography uses beads of a ____ compound.

____ chromatography uses a charged column and a variably saline eluent.

Ion-exchange chromatography uses a c____olumn and a variably saline eluent.

Size-exclusion chromatography relies on ____ beads.

____ chromatography relies on porous beads.

____ pores, in size-exclusion chromatography.

____ chromatography uses a bound receptor or ligand and an eluent with free ligand or a receptor for the protein of interest.

Affinity chromatography uses a ____ for the protein of interest.

Protein structure is primarily determined through ____ after the protein is isolated.

Protein ____ is primarily determined through X-ray crystallography after the protein is isolated.

Amino acid composition can be determined by ____.

Amino acid sequencing requires ____.

____ sequencing requires sequential degradation, such as the Edman degradation.

____d protein assay each test for protein.

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