Biochemistry

Chapter 3: Nonenzymatic Protein Function & Analysis

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____ proteins compose the cytoskeleton, anchoring proteins, and much of the extracellular matrix.

Structural proteins compose the ____.

The most common structural proteins are ____.

Structural proteins are generally ____ in nature.

____ proteins have one or more heads capable of force generation through a conformational change.

Motor proteins have catalytic activity, acting as ____ to power movement.

____ are the most common applications of motor proteins.

Common motor protein examples include ____.

____ allow cells to bind to other cells or surfaces.

____ are calcium-dependent glycoproteins that hold similar cells together.

Cadherins are ____ that hold similar cells together.

____ have two membrane-spanning chains and permit cells to adhere to proteins in the extracellular matrix.

Integrins have ____ and permit cells to adhere to proteins in the extracellular matrix.

Integrins have two membrane-spanning chains and permit cells to ____

____ allow cells to adhere to carbohydrates on the surfaces of other cells and are most commonly used in the immune system.

Selectins allow cells to adhere to carbohydrates on the surfaces of other cells and are most commonly used in the ____.

Selectins allow cells to adhere to ____ on the surfaces of other cells and are most commonly used in the immune system.

____ are used by the immune system to target a specific antigen.

Antibodies (or immunoglobulins, Ig) are used by the immune system to target a specific ____.

Immunoglobulins contain a ____ region.

The ____ region of antibodies is responsible for antigen binding.

The variable region of antibodies is responsible for ____.

Two identical ____ chains form a single antibody.

Light and heavy chains are held together by ____.

Three main types of ion channels are ____d channels.

____ ion channels are always open.

____ ion channels are open within a range of membrane potentials.

____ ion channels open in the presence of a specific binding substance.

____ participate in cell signaling through extracellular ligand binding and initiation of second messenger cascades.

Enzyme-linked receptors participate in cell signaling through ____.

____ have a membrane-bound protein associated with a trimeric G protein.

G protein-coupled receptors have a ____ associated with a trimeric G protein.

G protein-coupled receptors have a membrane-bound protein associated with a ____ protein.

G s protein ____

____ protein s timulates.

G i protein ____

____ protein i nhibits.

G q protein ____

____ protein activates p hospholipase C..

____ binding engages the G protein.

When ____ in a G protein cycle; the α subunit dissociates from the β and γ subunits.

When GDP is replaced with GTP in a G protein cycle; the ____ subunits.

The activated ____ subunit of G proteins alters the activity of adenylate cyclase or phospholipase C.

The activated α subunit of G proteins alters the activity of ____.

When ____; the α subunit rebinds to the β and γ subunits.

When GTP is dephosphorylated to GDP; the ____ subunits.

____ uses a gel matrix to observe the migration of proteins in response to an electric field.

Electrophoresis uses a ____ to observe the migration of proteins in response to an electric field.

Electrophoresis uses a gel matrix to observe the ____ in response to an electric field.

Electrophoresis uses a gel matrix to observe the migration of proteins in response to ____.

Native PAGE maintains the protein’s shape. ____

Native PAGE results are difficult to compare because ____.

SDS-PAGE ____ the proteins.

SDS-PAGE masks the ____ so that comparison of size is more accurate.

____ separates proteins by their isoelectric point (pI).

Isoelectric focusing separates proteins by their ____.

In Isoelectric focusing, the protein migrates toward an electrode until ____.

____ separates protein mixtures on the basis of their affinity for a stationary phase or a mobile phase.

Chromatography separates protein mixtures on the basis of ____.

____ uses beads of a polar compound.

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