Biochemistry

Chapter 2: Enzymes

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____ are biological catalysts that are unchanged by the reactions they catalyze and are reusable .

____ catalyze oxidation–reduction reactions that involve the transfer of electrons.

Oxidoreductases catalyze ____ reactions that involve the transfer of electrons.

Oxidoreductases catalyze oxidation–reduction reactions that involve ____.

____ move a functional group from one molecule to another molecule.

Transferases ____

____ catalyze cleavage with the addition of water.

Hydrolases ____

Lyases catalyze ____

____ catalyze cleavage without the addition of water and without the transfer of electrons.

____ catalyze the interconversion of isomers, including both constitutional isomers and stereoisomers.

Isomerases catalyze ____

____ are responsible for joining two large biomolecules, often of the same type.

Ligases are responsible for ____.

Exergonic reactions ____ energy.

____ reactions release energy.

Enzymes ____ the activation energy necessary for biological reactions.

Enzymes only change ____

Enzymes act by stabilizing the ____.

Enzymes have ____, which is the site of catalysis.

Binding to the active site is explained by the ____ model.

Some enzymes require metal cation ____ to be active.

____ is a regulatory mechanism whereby the catalytic activity of an enzyme is inhibited by the presence of high levels of a product later i…

Feedback inhibition is ____.

____ is characterized by the ability to replace the inhibitor with a compound of greater affinity or to remove it using mild laboratory tre…

Reversible inhibition is characterized by the ability to replace the inhibitor with ____.

____ results when the inhibitor is similar to the substrate and binds at the active site.

Competitive inhibition results when ____.

Competitive inhibition can be overcome by ____.

____ inhibition can be overcome by adding more substrate.

____ results when the inhibitor binds with equal affinity to the enzyme and the enzyme–substrate complex.

Noncompetitive inhibition results when ____.

____ results when the inhibitor binds with unequal affinity to the enzyme and the enzyme–substrate complex.

Mixed inhibition results when ____.

____ results when the inhibitor binds only with the enzyme–substrate complex.

Uncompetitive inhibition results when ____.

Competitive inhibition, the v max is ____.

____ inhibition, the v max is unchanged, K m increases.

Noncompetitive inhibition, the v max is ____.

____ inhibition, the v max is decreased, K m is unchanged.

Mixed inhibition, the v max is ____.

____ inhibition, the v max is decreased, K m is increased or decreased depending on if the inhibitor has higher affinity for the enzyme or…

Uncompetitive inhibition, the K m ____.

____ inhibition, the K m decreases and v max decreases.

____ sites can be occupied by activators, which increase either affinity or enzymatic turnover.

Allosteric sites can be occupied by ____, which increase either affinity or enzymatic turnover.

Allosteric sites can be occupied by activators, which increase either ____.

____ are secreted in an inactive form and are activated by cleavage.

Zymogens are secreted in an inactive form and are activated by ____.

Enzymes experience ____ kinetics.

Saturation kinetics states ____.

Michaelis–Menten and Lineweaver–Burk plots represent saturation kinetics relationship as a ____, respectively.

____ plots represent saturation kinetics relationship as a hyperbola and line, respectively.

Cooperative enzymes display a ____ curve.

____ enzymes display a sigmoidal curve.

Temperature and pH affect an enzyme’s activity in ____ .

____ affect an enzyme’s activity in vivo .

Salinity can impact the action of enzymes, in ____ .

____ can impact the action of enzymes, in vitro .

Tightly bound cofactors or coenzymes that are necessary for enzyme function are known as ____.